integrin αvβ3  (Bioss)

Journal: Molecular Imaging and Biology

Article Title: Quantifying Molecular Changes in the Preeclamptic Rat Placenta with Targeted Contrast-Enhanced Ultrasound Imaging

doi: 10.1007/s11307-025-01988-4

Figure Lengend Snippet: Quantification methods for binding contrast agent.​ a LE method: Acoustic intensity at t = t Burst – 40 s represents attached contrast agent value. b dTE method: Schematic showing changes before (t ≈ 2 min) and after Burst (t = t Burst + 30 s). The schematic illustrates the dTE method to quantify the attached contrast agent within the placenta. After tail vein injection, MBs adhere to the α ν β 3 integrin on the endothelial cells. Ten minutes later, a destructive ultrasound pulse is applied to destroy the adherent MBs, and 1 min later, the free-circulating MBs are replenished. c BCM method: data from t = 0 ~ 2 min are fitted into the complete equation for each pixel to calculate the binding constant of the attached MBs

Article Snippet: Placental sections were incubated with mouse polyclonal α ν β 3 integrin (1:200 dilution, Bioss antibodies, bs‐1310R) and secondary HRP-polymer (Rabbit-On-Rodent HRP-polymer, Biocare Medical, Pacheco, CA).

Techniques: Binding Assay, Injection

Journal: Molecular Imaging and Biology

Article Title: Quantifying Molecular Changes in the Preeclamptic Rat Placenta with Targeted Contrast-Enhanced Ultrasound Imaging

doi: 10.1007/s11307-025-01988-4

Figure Lengend Snippet: α ν β 3 integrin expression in NP vs RUPP placentas. a IHC staining in NP placenta. b IHC staining in RUPP placenta, showing decreased intensity. c Quantification of α ν β 3 integrin protein expression. Each data point represents the mean of 3–4 slices from a single placenta from each rat subject. ( n = 4 rats; mean ± SEM; * p < 0.05). d Relative α ν β 3 mRNA levels ( n = 6 each; normalized to β-Actin; mean ± SEM; ** p < 0.01)

Article Snippet: Placental sections were incubated with mouse polyclonal α ν β 3 integrin (1:200 dilution, Bioss antibodies, bs‐1310R) and secondary HRP-polymer (Rabbit-On-Rodent HRP-polymer, Biocare Medical, Pacheco, CA).

Techniques: Expressing, Immunohistochemistry